以CdTe/CdS量子点为荧光探针,荧光猝灭为理论基础,设计出一种快速、超灵敏且高选择性的检测片剂与注射剂中VB_1含量的方法。结果表明,VB_1在2.0×10~(-7)—5.0×10~(-5)mg/mL的范围内线性良好,相关系数为0.9994,检出限(S/N=3)为0.12ng/mL。将3×10~(-5)mg/mL的VB_1标准溶液平行测定7次,RSD为0.64%,加标回收实验结果满意。 Taking CdTe / CdS QDs as fluorescence probes and fluorescence quenching as the theoretical basis, a rapid, ultra-sensitive and highly selective method for the determination of VB_1 in tablets and injections was designed. The results showed that the linearity of VB_1 was good in the range of 2.0 × 10 -7 -5.0 × 10 -5 mg / mL, the correlation coefficient was 0.9994, the detection limit (S / N = 3) was 0.12 ng / mL. The 3 × 10 ~ (-5) mg / mL VB_1 standard solution was measured 7 times in parallel, with a RSD of 0.64%. The results of spike recovery experiments were satisfactory.