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建立一种新的检测嗜肺军团菌ｍｉｐ基因的引物双标记聚合酶链反应—酶联免疫吸附（ＰＣＲ－ＥＬＩＳＡ）法，应用于豚鼠军团菌感染的早期检测和诊断。其方法是将一对嗜肺军团菌引物分别标记有生物素和异硫氰酸荧光素，经ＰＣＲ扩增后，将标记有生物素的ＰＣＲ扩增产物加入链酶?
To establish a new primer pair for detecting Legionella pneumophila mip gene, double-labeled polymerase chain reaction-enzyme-linked immunosorbent assay (PCR-ELISA) was applied to early detection and diagnosis of Legionella infection in guinea pigs. The method is that a pair of pneumophila pneumophila primers are respectively labeled with biotin and fluorescein isothiocyanate, after PCR amplification, the biotin-labeled PCR amplification product is added into the chain enzyme?