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目的通过对儿童传染性单核细胞增多症(infectious mononucleosis,IM)外周血淋巴细胞亚群的检测,探讨其细胞免疫功能变化与疾病的关系。方法采用流式细胞仪对35例IM患儿外周血T、B和NK淋巴细胞亚群进行检测,30例健康儿童作为对照组;对患儿中的7例进行治疗2周后细胞亚群的测定以观察动态变化;对患儿进行外周血异型淋巴细胞计数。结果 IM患儿CD3、CD8 T淋巴细胞水平明显升高,CD19、NK、CD4和CD4/CD8值水平明显降低,分别与正常对照组比较差异均有统计学意义。7例IM患儿治疗2周后T细胞亚群的测定值与入院时比较差异有统计学意义,治疗前CD4、CD4/CD8值低于治疗后,治疗前CD8高于治疗后。IM患儿急性期CD8、CD4/CD8水平与患儿外周血异型淋巴细胞百分率(≤10%和>10%)的差异有统计学意义。结论检测淋巴细胞亚群对评估IM患儿的细胞免疫状况,辅助诊断和指导治疗具有重要的临床价值。
Objective To investigate the relationship between the change of cellular immune function and disease by detecting the peripheral blood lymphocyte subsets of infectious mononucleosis (IM) in children. Methods Flow cytometry was used to detect T, B and NK lymphocyte subsets in peripheral blood of 35 children with IM and 30 healthy children as control group. Seven of the children were treated for 2 weeks Determination to observe the dynamic changes in children with peripheral blood lymphocyte count. Results The levels of CD3 and CD8 T lymphocytes were significantly increased in IM children, and the levels of CD19, NK, CD4 and CD4 / CD8 were significantly lower in IM children than those in normal controls respectively. The measured value of T cell subsets in 7 IM patients after 2 weeks of treatment was significantly different from that at admission. The values of CD4 and CD4 / CD8 before treatment were lower than those after treatment, and CD8 before treatment was higher than that after treatment. There was significant difference in the percentages of CD8, CD4 / CD8 between the acute stage of IM children and the percentage of peripheral blood atypical lymphocytes (≤10% and> 10%) in children with IM. Conclusion Detection of lymphocyte subsets has important clinical value in assessing cellular immune status, adjuvant diagnosis and guiding therapy in children with IM.