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目的调查乌鲁木齐市2014年艾滋病病毒1型(HIV-1)耐药毒株的传播情况。方法参考世界卫生组织(WHO)的艾滋病传播性耐药调查方法,按确证时间顺序采集当年16~25岁新确证HIV-1感染者全血样本33份,分离血浆,提取病毒基因组RNA,扩增包含完整蛋白酶编码区和反转录酶1~250氨基酸基因区的pol基因片段并测序,利用Mega 6.0软件,通过与参考株pol序列构建进化树确定HIV-1亚型,pol序列与Stanford耐药数据库中的共享序列进行比对,利用WHO提供的CPR 5.0软件分析传播性耐药突变位点和耐药程度。结果检测的33份样本中成功获得序列的有32份,亚型以CRF07-BC(50.0%,16/32)为主,其次是CRF01-AE(34.4%,11/32)。32份样本中只有1份出现了耐药传播相关的突变位点,耐药传播率为3.1%(1/32)。结论乌鲁木齐市2014年的耐药传播水平较低,但应继续定期进行HIV-1耐药毒株传播的监测,了解HIV-1耐药毒株传播的动态变化。
Objective To investigate the transmission of HIV-1 resistant strains in Urumqi in 2014. Methods With reference to the World Health Organization (WHO) HIV / AIDS drug resistance survey, 33 samples of newly confirmed HIV-1 infected whole blood samples from 16 to 25 years of age were collected in a definite time sequence. Plasma was separated and the viral genomic RNA was extracted and amplified Pol gene fragment containing complete protease coding region and 1 ~ 250 amino acid region of reverse transcriptase was sequenced. Using Mega 6.0 software, the HIV-1 subtype was identified by constructing phylogenetic tree with pol sequence of reference strain. Pol sequence and Stanford resistance The shared sequences in the database were compared and analyzed using the CPR 5.0 software provided by the WHO for the analysis of transmission sites and drug resistance. RESULTS: Of the 33 samples tested, 32 were successfully obtained. The subtypes were CRF07-BC (50.0%, 16/32), followed by CRF01-AE (34.4%, 11/32). Only 1 out of 32 samples showed resistance-related mutation sites with a rate of 3.1% (1/32) of drug-resistant transmission. Conclusion The level of drug-resistant transmission in Urumqi was low in 2014, but the transmission of HIV-1 resistant strains should continue to be monitored periodically to understand the dynamic changes of HIV-1 resistant strains.