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目的:探讨慢性丙肝患者HCV感染与脂质代谢的关系及特点。方法:用酶联免疫法检测实验组慢性丙肝患者HCV不同基因片段抗体,并以羊抗人Apo-B血清分离其Apo-B,用PCR法检测Apo-B中HCV-RNA,同时作血脂监测。另设健康人和乙肝病例对照。结果:实验组病例HCV不同基因片段抗体呈现6种分布模式,其血清中均检出HCV-RNA较高载量,相应的Apo-B中(除1例阴性外)均有HCV-RNA表达,血清LDL、Apo-B等水平值显著低于对照组。两对照组血清及Apo-B中无HCV-RNA表达,且不同基因片段抗体阴性。结论:慢性丙肝患者HCV不同基因片段抗体应答与HCV复制密切相关,与Apo-B携带密切相关;其HCV-C和HCV-NS4、HCV-NS5与不同组分载脂蛋白相互作用,可能共同干扰脂质代谢。
Objective: To investigate the relationship between HCV infection and lipid metabolism in chronic hepatitis C patients. Methods: Antibody of HCV gene in chronic hepatitis C patients was detected by enzyme-linked immunosorbent assay (ELISA), Apo-B was isolated by goat anti-human Apo-B serum, HCV-RNA in Apo-B was detected by PCR, . Another set of healthy people and hepatitis B case control. Results: In the experimental group, there were 6 kinds of distribution patterns of antibodies in different HCV gene fragments. HCV-RNA was detected in all sera of patients in experimental group, and HCV-RNA was expressed in Apo-B (except 1 negative) Serum LDL, Apo-B levels were significantly lower than the control group. The two control serum and Apo-B did not express HCV-RNA, and different gene fragments were negative. CONCLUSIONS: The HCV antibody response in HCV patients with chronic hepatitis C is closely related to HCV replication and closely related to Apo-B carriage. The interaction of HCV-C, HCV-NS4 and HCV-NS5 with apolipoproteins may interfere fat metabolisim.