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目的探讨前列腺液半乳凝素3(Gal-3)浓度对诊断前列腺癌的临床价值。方法采用人Gal-3抗体免疫组化法对31例中老年男性前列腺穿刺组织进行染色,并按病理诊断分为前列腺癌组(A组,12例)和前列腺增生组(B组,19例)。应用多克隆抗体ELISA法检测前列腺液Gal-3浓度,分析两组Gal-3的表达差异。结果 A组前列腺液Gal-3浓度3703.3μg/ml,高于B组的1028.4μg/ml(P<0.01);A组前列腺组织Gal-3表达量亦大于B组(P<0.01)。4例前列腺癌患者合并转移性骨损害;其前列腺液Gal-3浓度高于8例非转移前列腺癌患者[(5265.6±345.2)μg/ml vs.(2292.1±1325.4)μg/ml](P<0.05)。取前列腺液Gal-3浓度为4167μg/ml时,其诊断前列腺癌的敏感度为100%,特异度为80%。结论前列腺液Gal-3检测可用于前列腺癌的诊断和是否发生骨转移的评估。
Objective To investigate the clinical value of prostatic fluid galectin-3 (Gal-3) in the diagnosis of prostate cancer. Methods Thirty-one middle-aged and old male prostate biopsies were stained with human Gal-3 antibody and divided into prostate cancer group (group A, n = 12) and benign prostatic hyperplasia group (group B, n = 19) . Polyclonal antibody was used to detect the concentration of Gal-3 in prostatic fluid, and the difference of Gal-3 expression between the two groups was analyzed. Results The concentration of Gal-3 in group A was 3703.3μg / ml, which was higher than that in group B (1028.4μg / ml) (P <0.01). The expression of Gal-3 in group A was also higher than that in group B (P <0.01). Four patients with prostate cancer had metastatic bone lesions, and the prostatic fluid Gal-3 concentration was higher than that of 8 patients with non-metastatic prostate cancer [(5265.6 ± 345.2) μg / ml vs. (2292.1 ± 1325.4) μg / ml] (P < 0.05). Prostate solution Gal-3 concentration of 4167μg / ml, the diagnosis of prostate cancer sensitivity was 100%, specificity of 80%. Conclusion Prostate fluid Gal-3 test can be used for the diagnosis of prostate cancer and the assessment of bone metastases.