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甘油-3-磷酸酰基转移酶(Glycerol-3-phosphate acyltransferases,GPATs;EC 2.3.1.15)是植物种子中储藏性油脂三脂酰甘油(Triacylglycerols,TAGs)合成中的一个关键酶,它催化甘油-3-磷酸分子sn-1位的酰化反应,形成溶血磷酯酸(Lysophosphatidic acid,LPA),从而起动TAGs的生物合成。拟南芥内质网中表达的AtGPAT9在此过程中可能起着重要作用。运用RT-PCR方法克隆到AtGPAT9基因的编码区cDNA,并构建了其植物表达载体,然后通过农杆菌介导的子叶节法对大豆进行了遗传转化,获得抗草丁膦(Phosphinothricin/PPT)筛选剂的再生植株。
Glycerol-3-phosphate acyltransferases (GPATs; EC 2.3.1.15) are key enzymes involved in the synthesis of the storage oil triglycerides (TAGs) in plant seeds. They catalyze the synthesis of glycerol- The acylation of the 3-phosphate molecule at the sn-1 position results in the formation of Lysophosphatidic acid (LPA), which initiates the biosynthesis of TAGs. AtGPAT9 expressed in the Arabidopsis endoplasmic reticulum may play an important role in this process. The coding region cDNA of AtGPAT9 gene was cloned by RT-PCR and its plant expression vector was constructed. Then the soybean was transformed by Agrobacterium tumefaciens-mediated cotyledonary node method, and the screening of Phosphinothricin / PPT Regenerated plants.