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目的：建立一种检测蚊体内马来丝虫幼虫的灵敏、快速、特异的方法。方法：选择应用ＰＣＲ及ＰＣＲ－ＥＬＩＳＡ法检测马来丝虫幼虫ＤＮＡ的最佳反应条件，并在该条件下分别以马来丝虫Ⅰ期、Ⅱ期、Ⅲ期幼虫各１条作模板，测定检测的灵敏度及检测实验室人工感染中华按蚊体内的马来丝虫幼虫。结果：ＰＣＲ及ＰＣＲ－ＥＬＩＳＡ法均能检测出１条Ⅰ期幼虫（Ｌ１），而ＰＣＲ的检测下限为１／１０条Ｌ１，ＰＣＲ－ＥＬＩＳＡ检测下限为１／１００条Ｌ１；将分离的感染期幼虫加阴性蚊媒进行粗提、扩增及电泳，结果未见明显的扩增条带，扩增产物作ＥＬＩＳＡ检测，全部为阴性；个体解剖人工感染的中华按蚊１２０只，分别收集１１３只阳性蚊体内的幼丝虫，用两种方法检测，结果均为阳性。结论：初步建立了ＰＣＲ及ＰＣＲ－ＥＬＩＳＡ法检测蚊体内马来丝虫幼虫的方法。
Objective: To establish a sensitive, rapid and specific method for detecting larvae of malaria in mosquitoes. Methods: The optimum reaction conditions of DNA of larvae of larvae were tested by PCR and PCR-ELISA. One of the larvae of stage Ⅰ, Detection sensitivity and detection laboratory artificial infection of Anopheles sinensis body larvae. Results: One stage larvae (L1) was detected by PCR and PCR-ELISA, respectively. The detection limit was 1/10 for PCR and 1/100 for PCR-ELISA. The infection period Larvae and negative mosquito vectors for crude extraction, amplification and electrophoresis, the results showed no significant amplification bands, amplification products were detected by ELISA, all negative; individual anatomy artificial infection of Anopheles sinensis 120, were collected 113 Positive mosquitoes within the young filariasis, detected by two methods, the results were positive. Conclusion: The method of PCR and PCR-ELISA for the detection of larvae of malayian larvae in mosquitoes was preliminarily established.