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目的观察β-七叶皂苷钠对大鼠脑出血后脑水肿的影响。方法用立体定位法采用新鲜自体血注入大鼠尾状核建立脑出血模型,将实验大鼠随机分为治疗组和对照组;根据用药时间,两组各分成1、3、6、24 h组,治疗组于相应时间点经尾静脉注射七叶皂苷钠(2.5 mg/kg.d),对照组于相应时间点连续3天尾静脉注射生理盐水。干湿重法观察大鼠脑含水量以及血肿周围组织肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)、基质金属蛋白酶-9(MMP-9)的表达。结果治疗组脑含水量明显低于对照组(P<0.05),各治疗组间相比差异无显著性(P>0.05)。对照组免疫荧光染色示TNF-α在出血灶周围组织广泛表达,而治疗组阳性细胞表达显著低于对照组(P<0.05),血肿周围组织IL-6表达与对照组相比差异无显著性(P>0.05),对照组MMP-9的表达显著高于治疗组(P<0.05)。结论β-七叶皂苷钠有抗脑出血后脑水肿的作用,其作用途径可能通过抗炎性损伤来实现。
Objective To observe the effect of sodium β-aescin on cerebral edema after intracerebral hemorrhage in rats. Methods Intracerebral hemorrhage models were established by stereotaxic method using fresh autologous blood injection into rat caudate nucleus. The experimental rats were randomly divided into treatment group and control group. According to the time of administration, the two groups were divided into 1, 3, 6, 24 h group. In the treatment group, sodium aescinate (2.5 mg/kg.d) was injected via the tail vein at the corresponding time points, and the control group was injected intravenously with physiological saline for 3 consecutive days at the corresponding time points. The brain water content and the expression of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and matrix metalloproteinase-9 (MMP-9) around the hematoma were observed by dry-wet weight method. Results The brain water content in the treatment group was significantly lower than that in the control group (P<0.05). There was no significant difference between the treatment groups (P>0.05). The immunofluorescence staining of control group showed that TNF-α was widely expressed in the tissues around the hemorrhage, but the expression of positive cells in the treatment group was significantly lower than that in the control group (P<0.05). The expression of IL-6 in the surrounding tissue of the hematoma was not significantly different from the control group. (P>0.05), the expression of MMP-9 in the control group was significantly higher than that in the treatment group (P<0.05). Conclusion β-aescin has anti-cerebral edema effect after cerebral hemorrhage, and its action may be achieved through anti-inflammatory injury.