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目的 :观察腹腔注射脂多糖诱导小鼠内毒素性全身炎症反应综合征和多器官功能不全综合征的作用。方法 :选用 5～ 7周雄性BALB/c小鼠 ,腹腔注射不同剂量大肠杆菌脂多糖 ,观察小鼠一般状态、死亡率、肛温及脏器的病理变化 ,并重点观察了 6mg/kgLPS诱导小鼠TNF α分泌的作用及其对小鼠代谢和器官功能的影响。结果 :腹腔注射LPS可致小鼠出现腹泻、紫绀、竖毛等症状 ,肛温和死亡率变化呈LPS剂量依赖性。LPS对小鼠的半数致死量为 9 0mg/kg。 6 0mg/kg的LPS剂量可使小鼠代谢紊乱 ,表现为血糖降低 ,血脂及载脂蛋白水平升高 ;可使肺、小肠、心、肾、肝等器官损伤 ,表现为血清胆红素、谷草转氨酶、磷酸肌酶、尿酸水平升高 ,病理形态学表现为充血、水肿、炎细胞浸润甚至细胞坏死。受累的脏器数随LPS剂量的增加而增加。结论 :腹腔注射LPS (6 0mg/kg)可致小鼠SIRS和MODS ,9 0mg/kg体重LPS为其半数致死剂量。
Objective: To observe the effect of intraperitoneal injection of lipopolysaccharide (LPS) on endotoxin-induced systemic inflammatory response syndrome and multiple organ dysfunction syndrome in mice. Methods: Male BALB / c mice (5 to 7 weeks old) were injected intraperitoneally with different doses of lipopolysaccharide (LPS) to observe the general status, mortality, rectal temperature and organ pathological changes in mice. Effect of murine TNFα secretion on metabolism and organ function in mice. Results: Intraperitoneal injection of LPS caused diarrhea, cyanosis and piloerection in mice. The changes in rectal temperature and mortality were dose-dependent in LPS. The lethal dose of LPS to mice was 90 mg / kg. LPS dose of 60mg / kg can make mice metabolic disorders, manifested as hypoglycemia, elevated blood lipids and apolipoprotein levels; can lung, small intestine, heart, kidney, liver and other organ damage, manifested as serum bilirubin, Aspartate aminotransferase, phosphokinase, uric acid levels, pathological manifestations of congestion, edema, inflammatory cell infiltration or even cell necrosis. The number of organs involved increased with increasing doses of LPS. Conclusion: Intraperitoneal injection of LPS (60mg / kg) can induce SIRS and MODS in mice and LPS at 90mg / kg is the lethal dose.