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目的:观察调经助孕方对雄激素致不孕大鼠排卵的促进作用。方法:采用9 d龄雌性大鼠注射丙酸睾丸酮建立雄激素致不孕大鼠模型,随机分为模型组及中药组,并另设正常对照组;自81 d龄起,中药组灌胃调经助孕方,模型组与正常组灌胃等量蒸馏水,连续30 d。采用阴道涂片测定大鼠动情周期及排卵率,采用ELISA法测定卵泡刺激素(FSH)、黄体生成素(LH)、睾酮(T)、雌激素(E2)、脱氢表雄酮(DHA)。结果:模型组大鼠表现为动情间期延长,且丧失正常性周期;中药组动情周期出现周期性变化,排卵恢复率为66.7%,两组差异有统计学意义(P<0.05)。模型组大鼠FSH、LH和E2含量明显低于正常组(P<0.05),DHA、T含量明显高于正常组(P<0.05);与模型组比较,中药组大鼠FSH、LH和E2含量明显升高(P<0.05),DHA、T含量明显降低(P<0.05)。结论:调经助孕方能够明显提高雄激素致不孕大鼠的排卵率,改善大鼠生殖内分泌轴功能。
Objective: To observe the effects of regulating menstruation and promoting pregnancy on ovulation in androgen-sterilized rats. Methods: 9-day-old female rats injected with testosterone propionate to establish androgen-sterilized rat model, were randomly divided into model group and traditional Chinese medicine group, and another set of normal control group; since 81 d age, Pregnancy, model group and normal group were orally administered with distilled water for 30 days continuously. The estrous cycle and ovulation rate of rats were measured by vaginal smear. The levels of follicle stimulating hormone (FSH), luteinizing hormone (LH), testosterone (T), estrogen (E2), dehydroepiandrosterone (DHA) . Results: The rats in the model group showed prolonged estrous interval and lost the normal cycle. The estrous cycle of the traditional Chinese medicine group showed periodic changes with an ovulation recovery rate of 66.7%. There was significant difference between the two groups (P <0.05). The contents of FSH, LH and E2 in the model group were significantly lower than those in the normal group (P <0.05), and the levels of DHA and T in the model group were significantly higher than those in the normal group (P <0.05) (P <0.05), while the content of DHA and T decreased significantly (P <0.05). Conclusion: Tiaojing Zhuyun Recipe can significantly increase the ovulation rate of male sterility induced by androgen and improve the reproductive and endocrine axis function in rats.