实验性动脉粥样硬化内皮屏障功能的研究,多年来一直颇受重视。其中如何选择和建立一个能较好反映生体内皮通透性的检查方法是十分重要的。为此,本文重点探讨了Evans蓝活体染色的用量、方法,并确定了肉眼观察与荧光显微镜观测相结合的判断标准。通过我们在实验性动粥研究中应用,证实了此法简便,效果稳定,而且对内皮通透性改变的 Experimental atherosclerotic endothelial barrier function has been the subject of considerable attention over the years. Among them, how to choose and establish a check method that can better reflect the permeability of the body’s endothelium is very important. To this end, this article focuses on the amount of Evans blue staining, the method, and determine the combination of macroscopic observation and fluorescence microscopy criteria. Through our application in the experimental porridge research, this method was proved to be simple, stable, and the change of endothelial permeability