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目的 研究内皮抑素对胃癌生长和转移的抑制作用 ,并探讨其对胃癌细胞凋亡的影响。方法 建立人胃腺癌裸鼠原位种植转移模型。将 72只荷瘤裸鼠随机分成 4组 ,对照组 3 6只 ,治疗各组每组 12只。种植后第 1周开始皮下注射内皮抑素 ,隔天 1次 ,剂量为 0mg/kg(对照组 )、2 5mg/kg、10 0mg/kg、2 0 0mg/kg(治疗组 ) ,共用 7周。种植后第 8周处死动物 ,测量原位肿瘤体积、抑瘤率、肿瘤微血管密度 (MVD)、肿瘤细胞凋亡指数 (AI) ,观察肿瘤细胞腹膜、肝、其他脏器转移及腹水情况。结果 内皮抑素剂量为 0mg/kg、2 5mg/kg、10 0mg/kg、2 0 0mg/kg时 ,原位肿瘤体积分别为 ( 15 83±5 76)mm3、( 5 91± 3 84 )mm3、( 65 7± 4 3 1)mm3、( 1 89± 1 0 2 )mm3;抑瘤率分别为 0、62 7%、95 8%、99 9% ;MVD分别为 ( 13 70± 3 90 )、( 5 73± 2 3 6)、( 2 17± 1 2 8)、( 0 66± 0 2 5 ) ;AI分别为 ( 3 91±2 5 8) %、( 6 76± 5 0 3 ) %、( 18 92± 6 75 ) %、( 2 8 5 7± 10 3 4 ) % ;腹膜转移率分别为 87 1%、5 4 5 %、16 7%、0 ;肝转移率分别为 83 9%、2 7 3 %、8 3 %、0。治疗组与对照组相比 ,组间胃癌生长和转移的抑制作用差异有显著性意义 (t=3 1 77,P <0 0 5 ) ,且抑制作用与内皮抑素?
Objective To study the inhibitory effect of endostatin on the growth and metastasis of gastric cancer, and to explore its effect on gastric cancer cell apoptosis. Methods A model of human gastric adenocarcinoma in nude mice was established. 72 nude mice were randomly divided into 4 groups, 36 in the control group and 12 in each group. Endosteone was subcutaneously injected 1 week after implantation, every other day, at doses of 0 mg/kg (control), 25 mg/kg, 100 mg/kg, and 200 mg/kg (treatment group) for 7 weeks. . Eight weeks after implantation, the animals were sacrificed and the in situ tumor volume, tumor inhibition rate, tumor microvessel density (MVD) and tumor cell apoptosis index (AI) were measured. The peritoneal, liver, and other organ metastases and ascites of the tumor cells were observed. Results When the endostatin doses were 0 mg/kg, 25 mg/kg, 100 mg/kg, and 200 mg/kg, the orthotopic tumor volume was (15 83±5 76) mm 3 and (5 91± 3 84) mm 3, respectively. The tumor inhibition rates were 0, 62 7%, 95 8%, and 99 9%, respectively; and the MVDs were ( 13 70± 3 90). (5 73 ± 2 3 6), (2 17 ± 1 2 8), (0 66 ± 0 2 5); AI is (3 91 ± 2 5 8) %, ( 6 76 ± 5 0 3 ) % respectively. (18 92± 6 75)%, (2 8 5 7± 10 3 4) %; peritoneal metastasis rates were 87 1%, 5 4 5 %, 16 7%, 0 respectively; liver metastases were 83 9% respectively. , 2 7 3 %, 8 3 %, 0. Compared with the control group, there was a significant difference in the inhibitory effect of the growth and metastasis of gastric cancer between the treatment group and the control group (t = 37 77, P < 0 05), and the inhibitory effect with endostatin?