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作者用几种增加或减少红细胞内、外游离钙技术,观察了钙在恶性疟原虫入侵人红细胞过程中的作用。实验用泰国恶性疟原虫T_9分离株的克隆T_(9/960)用Percoll进行等密度离心分离裂殖体,加入含10%人血清和A型人红细胞的RPMI-1640培养基中连续培养,经4h虫体再侵入新的红细胞后,残存的裂殖体用山梨醇溶解,如此反复3次使虫体培养同步化而获得纯化裂殖体。此外,以PBS洗涤红细胞
The authors used several methods to increase or decrease intracellular and extracellular free calcium in erythrocytes and observed the role of calcium in the inactivation of human erythrocytes by Plasmodium falciparum. In the experiment, clone T_ (9/960) of P. thaliana T_9 isolate was isolated by iso-density centrifugation using Percoll and then cultured in RPMI-1640 medium supplemented with 10% human serum and type A human erythrocytes. 4h after invade new red blood cells, the remaining schizonts dissolved with sorbitol, so repeated 3 times to synchronize the parasite culture to obtain purified schizonts. In addition, erythrocytes were washed with PBS