本研究在前期研究基础上,采用改良RACE技术和RT-PCR技术克隆了一个杧果Mn-MiSOD基因的全长序列。生物信息学分析显示,该cDNA全长为794 bp,包含一个690 bp的开放阅读框,编码230个氨基酸,分子量为25.67 kDa,等电点为6.3。表达模式分析显示,该基因在各个组织中均表达,但在成熟叶片和果实中表达水平更高。低温胁迫、盐胁迫和PEG干旱胁迫均诱导该基因的表达。热处理、柠檬酸处理、草酸处理、1-MCP处理以及热处理+1-MCP处理上调Mn-MiSOD基因在果实中的表达水平。实验结果表明,Mn-MiSOD基因可能在杧果逆境胁迫和采后生理方面起重要作用。 Based on the previous studies, we cloned the full-length sequence of Mn-MiSOD gene from mango using modified RACE and RT-PCR techniques. Bioinformatics analysis showed that the cDNA was 794 bp in length and contained a 690 bp open reading frame encoding 230 amino acids with a molecular weight of 25.67 kDa and an isoelectric point of 6.3. Expression pattern analysis showed that the gene was expressed in all tissues but higher in mature leaves and fruits. Low temperature stress, salt stress and PEG drought stress all induced the gene expression. Heat treatment, citric acid treatment, oxalic acid treatment, 1-MCP treatment and heat treatment + 1-MCP treatment up-regulated the expression level of Mn-MiSOD gene in fruits. The experimental results show that Mn-MiSOD gene may play an important role in stress and postharvest physiology.