目的　在酵母中实现人血管内皮生长因子 (hVEGF165)受体FLT 1胞外 1 3及 2 3loopcDNA的高效分泌性表达 ,并比较两种表达产物的生物学活性。方法　构建酵母重组表达质粒pPIC9K/FLT 1(1 3)及pPIC9K/FLT 1(2 3) ,并分别转化酵母宿主菌GS115。表达产物经CM SepharoseFF阳离子交换层析和SephacrylS 10 0分子筛层析纯化后测定其生物学活性。结果　SDS PAGE显示 ,表达产物以可溶性分子形式存在于上清中 ,诱导 4d的表达量占上清总蛋白的 6 0 %以上。表达的sFLT 1(1 3)及sFLT 1(2 3)都具有结合hVEGF165的能力和抑制hVEGF165对人脐静脉内皮细胞 (HUVEC)的促增殖功能 ,其中前者的作用虽较强 ,但二者相差不大。结论　获得了具有生物学活性的可溶性重组FLT 1受体胞外区 1 3及 2 3loop小片段 ,为进一步的动物实验和临床应用提供有益的资料 OBJECTIVE: To efficiently and efficiently express human vascular endothelial growth factor (hVEGF165) receptor FLT 1 extracellular 1 3 and 2 3 loop cDNA in yeast and compare the biological activities of the two expressed products. Methods Recombinant yeast plasmids pPIC9K / FLT1 (1 3) and pPIC9K / FLT1 (2 3) were constructed and transformed into yeast host strain GS115 respectively. The expressed product was purified by CM Sepharose FF cation exchange chromatography and Sephacryl S 10 0 molecular sieve chromatography to determine its biological activity. Results SDS PAGE showed that the expressed product was soluble in the supernatant in the form of soluble molecules, and the expression level of the expressed protein was over 60% of the total protein in the supernatant induced by SDS PAGE. The expressed sFLT 1 (1 3) and sFLT 1 (2 3) both had the ability to bind to hVEGF165 and inhibit the proliferation of human umbilical vein endothelial cells (HUVEC) induced by hVEGF165, but the difference between the two Not big. Conclusions The bioactive small fragments of extracellular domain 1 3 and 2 3loop of recombinant FLT 1 receptor were obtained and provided useful data for further animal experiments and clinical application