论文部分内容阅读
目的:通过建立通关藤药材C21甾体皂苷类成分的特征图谱,为科学评价药材质量提供理论依据。方法:色谱条件:Sinochrom ODS-BP分析柱(4.6 mm×250 mm,5μm),柱温室温,检测波长223 nm,流动相乙腈-0.1%磷酸水溶液(32∶68～85∶15)梯度洗脱,流速1 mL.min-1。结果:通关藤药材指纹图谱选定了17个共有峰,其中2号峰为通关藤苷A,建立了通关藤药材HPLC指纹图谱共有模式,得到不同产地通关藤药材的相似度数据。结论:建立的指纹图谱重复性好,具有较广泛的应用范围,可用于通关藤药材的HPLC指纹图谱评价其质量。
OBJECTIVE: To provide a theoretical basis for scientifically evaluating the quality of medicinal materials by establishing a characteristic map of the steroidal saponins constituents of C21. Methods: Chromatographic conditions were as follows: Sinochrom ODS-BP analytical column (4.6 mm × 250 mm, 5 μm), column temperature at room temperature, detection wavelength of 223 nm and gradient elution with mobile phase of acetonitrile-0.1% phosphoric acid , Flow rate 1 mL.min-1. Results: Seventeen common peaks were identified by fingerprinting of Radix Et Rhizoma. The peak of No.2 was stannous glycoside A. The common fingerprint of Radix et Rhizoma Rhei was established, and the similarity data of Radix et Rhizoma Rhei was obtained. Conclusion: The established fingerprint has good reproducibility and has a wide range of applications. It can be used to evaluate the quality of HPLC fingerprints of Radix et Rhizoma.