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从发生叶斑病的牛角瓜病叶片上分离到1株真菌,菌株编号记为CX008;通过传统的形态学观察与现代分子生物学技术相结合的手段进行了病原菌鉴定,并依据其形态特点对其培养性状进行了初步研究,同时研究了不同杀菌剂对菌落生长的影响。结果表明:该病原菌为丝孢纲(Hyphomycetes)丝孢目(Hyphomycetales)暗色孢科(Dematiaceae)交链孢属(Alternaria)细极链格孢络石生变种[Alternaria tenuissima(Nees ex Fr.)];该菌对供试的7种氮源和碳源均能利用,最佳碳源和氮源分别为葡萄糖和牛肉膏;该病原菌在15~40℃之间均能生长,最适的生长温度是25℃和30℃,在5℃、10℃和45℃条件下停止生长;该病原菌孢子在15~40℃之间均能萌发,最适萌发温度为28℃,高于40℃孢子无法萌发;12h/12h光暗交替有利于菌落生长,有无光照对分生孢子萌发没有明显地影响;5种广谱杀菌剂对该菌落的生长均有抑制作用,扑海因对该菌的抑制效果最好,随后依次是恶霜锰锌、百菌清、代森锰锌,效果最差的是敌克松。
One strain of fungus was isolated from leaves of melon and melon with leaf spot disease. The number of the strain was recorded as CX008. Pathogen identification was carried out by traditional morphological observation combined with modern molecular biology techniques. According to its morphological characteristics, The culture traits were studied preliminarily, and the effects of different fungicides on colony growth were also studied. The results showed that the pathogen was Alternaria tenuissima (Nees ex Fr.) from Hyphomycetes (Alternaria tenuissima) of Alternaria (Dematiaceae) The bacteria could be used for the seven nitrogen and carbon sources tested, the best carbon source and nitrogen source were glucose and beef extract, respectively. The pathogen could grow between 15 ~ 40 ℃, and the optimum growth temperature was 25 ℃ and 30 ℃, and stopped growing at 5 ℃, 10 ℃ and 45 ℃. The spore of the pathogen could germinate at the temperature between 15 ℃ and 40 ℃, the optimum temperature was 28 ℃, and the spore above 40 ℃ could not germinate. 12h / 12h light and dark alternate conducive to colony growth, with or without light on conidia germination had no significant effect; 5 kinds of broad-spectrum fungicide on the growth of the colony were inhibited, the inhibitory effect of fluhexin on the bacteria Well, followed by a frost Mn-Zn, chlorothalonil, mancozeb, the worst is the enemy Kesi.