建立从尖吻蝮蛇毒中分离纯化38k凝血酶样酶的生产工艺,利用离子交换和凝胶过滤技术,中间品加入巯基乙醇处理后,再利用凝胶过滤技术除去性质相近的杂蛋白,获得的38k凝血酶样酶HPLC纯度达99.93%,比活力达4416U/mg,出血毒试验800U/ml无出血,所建立的分离纯化方法适合规模生产。 The production process of 38k thrombin-like enzyme was purified from Agkistrodon acutus venom. Ion exchange and gel filtration were used to treat the intermediate protein with mercaptoethanol, then the gel filtration technique was used to remove the hybrid protein with similar properties. 38K thrombin-like enzyme HPLC purity of 99.93%, specific activity of 4416U / mg, bleeding test 800U / ml no bleeding, the established separation and purification method for mass production.