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目的:探讨加味四逆散对异相睡眠剥夺168 h大鼠的学习记忆能力及前额叶皮质突触结构可塑性的作用。方法:以多平台法复制睡眠剥夺模型,药物干预组以加味四逆散灌胃,分别在睡眠剥夺6,30,54,78,102,126,150 h时间点给药,每次用药量为16.25 g.kg-1。应用Y迷宫评估动物学习与记忆能力的变化,并使用透射电镜观察动物前额叶皮质(prefrontalcortex,PFC)突触结构可塑性的改变。结果:加味四逆散组大鼠Y迷宫成绩明显高于模型组大鼠(P<0.05)。与模型组比较,加味四逆散组大鼠PFC神经元突触间隙明显变窄(P<0.05),突触后膜致密物(post synaptic density,PSD)厚度明显变厚(P<0.01)。结论:加味四逆散能改善异相睡眠剥夺模型大鼠学习与记忆能力,与其影响前额叶皮质神经元突触结构可塑性,从而增强其突触传递效能有关。
Objective: To investigate the effect of Jiawei Sini Decoction on the learning and memory abilities and synaptic plasticity of prefrontal cortex in rats exposed to different phases of sleep deprivation for 168 h. Methods: The sleep deprivation model was replicated by multi-platform method. The drug intervention group was given Jiawei Sini Decoction intragastrically, and were dosed at 6,30,54,78,102,126,150 h after sleep deprivation, each dosage was 16.25 g.kg-1 . The Y maze was used to evaluate the changes of learning and memory ability in animals. Transmission electron microscopy was used to observe the synaptic plasticity of prefrontal cortex (PFC). Results: The Y maze scores of rats in Jiawei Sini San group were significantly higher than those in model group (P <0.05). Compared with the model group, the synaptic clearance of PFC neurons in rats in Jiawei Sini San group was significantly narrower (P <0.05) and the thickness of postsynaptic density (PSD) thicker (P <0.01). Conclusion: Jiawei Sini Decoction can improve the ability of learning and memory in rats with heterogenous sleep deprivation, which may be related to its influence on the plasticity of synaptic structure in prefrontal cortex neurons and the enhancement of synaptic transmission.