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目的分析不同脱钙条件对骨组织免疫组织化学(免疫组化)染色抗原性的影响。方法用8%硝酸分别于微波、光波及光波+微波组合Ⅰ、光波+微波组合Ⅱ的条件下对骨组织进行脱钙,并将室温(20℃)条件下脱钙的骨组织作为对照组,运用链霉菌素卵白素-生物素(LSAB)免疫组化染色技术,并对其染色效果进行分析比较。结果微波、光波、光波+微波组合Ⅰ及光波+微波组合Ⅱ的脱钙时间显著低于对照组,且免疫组化染色效果显著优于对照组(P<0.05);光波+微波组合的免疫组化染色效果显著优于微波、光波(P<0.05);但微波与光波之间、光波+微波组合Ⅰ与光波+微波组合Ⅱ之间的免疫组化染色效果比较,差异无统计学意义(P>0.05)。结论光波+微波组合脱钙时间要明显短于微波、光波及室温脱钙,且具有良好的免疫组化染色效果。
Objective To analyze the effect of different decalcification conditions on the immunohistochemical (immunohistochemical) staining of bone tissue. Methods Bone tissue was decalcified with 8% nitric acid under the condition of microwave, light wave and light wave + microwave combination Ⅰ, light wave + microwave combination Ⅱ, and the decalcified bone tissue under room temperature (20 ℃) The streptavidin-biotin (LSAB) immunohistochemical staining technique was used, and the dyeing effect was analyzed and compared. Results The decalcification time of microwave, light wave, light wave + microwave combination Ⅰ and light wave + microwave combination Ⅱ was significantly lower than that of the control group, and the immunohistochemical staining effect was significantly better than that of the control group (P <0.05) (P <0.05). However, there was no significant difference in the immunohistochemical staining results between microwave and light wave, light wave + microwave combination Ⅰ and light wave + microwave combination Ⅱ (P > 0.05). Conclusion The combination of light wave and microwave decalcification time is significantly shorter than microwave, light wave and room temperature decalcification, and has good immunohistochemical staining results.