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中国仓鼠卵巢细胞(CHO细胞)系是真核表达系统,其制备重组乙型肝炎(乙肝)疫苗的发展前景较之原核的酵母细胞系更好。该文综述了国产重组乙肝疫苗(CHO细胞)的临床效果,认为重组乙肝疫苗(CHO细胞)具有良好的安全性;无论是新生儿、儿童或是成人易感者接种3剂重组乙肝疫苗(CHO细胞)后,乙肝病毒表面抗体(抗-HBs)阳转率都可达到90%以上,抗体几何平均浓度(GMC)约为200毫国际单位/毫升(mIU/ml);以20μg/ml剂量为乙肝病毒表面抗原(HBsAg)和乙肝病毒e抗原(HBeAg)双阳性母亲的新生儿做母婴阻断,阻断率可达90%以上;将重组乙肝疫苗(CHO细胞)用于人群乙肝免疫预防,可将新一代人的HBsAg阳性率降到1%以下。这些研究结果提示,重组乙肝疫苗(CHO细胞)的效果与重组乙肝疫苗(酵母)相当,可用于中国的乙肝预防。
Chinese hamster ovary cells (CHO cells) are eukaryotic expression systems, and their development prospects for recombinant hepatitis B (hepatitis B) vaccines are better than prokaryotic yeast cell lines. This review summarizes the clinical efficacy of domestic recombinant hepatitis B vaccine (CHO cells) and concludes that recombinant hepatitis B vaccine (CHO) has good safety. Neonatal, pediatric or adult susceptible patients are vaccinated with three doses of recombinant hepatitis B vaccine (CHO Cells), the positive rate of HBsAg could reach more than 90%, and the antibody geometric mean concentration (GMC) was about 200 milliunits per milliliter (mIU / ml). At the dose of 20μg / ml Hepatitis B virus surface antigen (HBsAg) and hepatitis B virus e antigen (HBeAg) double positive mothers neonatal maternal and child block, the blocking rate of up to 90%; the recombinant hepatitis B vaccine (CHO cells) for the population of hepatitis B immune prevention , A new generation of HBsAg-positive rate can be reduced to below 1%. These findings suggest that the effect of recombinant hepatitis B vaccine (CHO cells) is comparable to recombinant hepatitis B vaccine (yeast) and can be used for hepatitis B prevention in China.