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为系统揭示黄连木天然居群在遗传多样性水平上的差异及遗传分化状况,采用SSR(Simple sequence repeats)分子标记对其8个居群进行遗传多样性分析.在建立良好的反应体系基础上,从已有的阿月浑子SSR引物中筛选出9对适合进行黄连木遗传分析的引物,在8个黄连木居群中共检测到43条等位基因,位点平均等位基因数为4.78,平均多态位点百分率达90.28%.各居群内平均有效等位基因数为2.08,平均期望杂合度(He)为0.472,说明各居群的遗传多样性处于中等水平.按检测到的有效等位基因数(Ne)和期望杂合度(He),各居群的遗传多样性由高至低依次为安康>唐县>顺平>辉县>略阳>栾川>林州>涉县.居群间的遗传分化系数(FST)平均为0.319,说明居群内变异是黄连木变异的主要来源,并根据遗传距离将8个居群分为三大类.
In order to reveal the genetic diversity and genetic differentiation of natural populations of Pistacia chinensis, the genetic diversity of eight populations of Pistacia chinensis was analyzed by using simple sequence repeats (SSR) markers.Based on the establishment of a good reaction system , 9 primers suitable for the genetic analysis of Pistacia chinensis were screened from the existing Pistachio SSR primers. A total of 43 alleles were detected in 8 populations of Pistacia chinensis, with an average number of alleles of 4.78 , The average percentage of polymorphic loci was 90.28% .The average number of effective alleles in each population was 2.08, and the average expected heterozygosity (He) was 0.472, indicating that the genetic diversity of each population was at a moderate level.According to the detected The number of effective alleles (Ne) and expected heterozygosity (He), and the genetic diversity of each population from high to low were Ankang> Tangxian> Shunping> Huixian> Lueyang> Luanchuan> Linzhou> The average coefficient of genetic differentiation (FST) among populations in the counties and counties was 0.319, which indicated that intra-population variation was the main source of variation in Pistacia chinensis, and the eight populations were divided into three categories based on genetic distance.