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收集罗氏沼虾肌肉白浊病典型病虾苗 ,进行细菌分离、除菌组织悬液制备及人工感染试验、超薄切片、病毒粗提取 ,结果表明 :从白浊病病虾的肌肉、肝胰腺中分离到的细菌以5× 1 0 8CFU/mL浓度浸泡感染正常沼虾苗不能复制疾病 ;病虾除菌组织过滤液以 1∶5 0、1∶2 5 0浓度浸泡感染正常虾苗 ,7d后可发病 ,并出现典型的肌肉白浊病症状 ,氯仿处理不能破坏病毒的感染力 ;病虾超薄切片电镜观察发现肌肉间隙组织中存在 2 1— 2 3nm大小的球状病毒 ,病虾组织浆经氯仿处理、PEG沉淀、磷钨酸负染后可见到大量 2 2— 2 4nm大小的球状病毒颗粒。上述结果表明罗氏沼虾肌肉白浊病是一种由病毒引起的疾病。
Macrobrachium rosenbergii muscle shrimp were collected typical disease shrimp, bacterial isolation, bacterial suspension preparation and artificial infection test, ultra-thin sections, the crude virus extract, the results show that: from white turbid disease shrimp muscle, liver and pancreas Bacteria isolated at 5 × 10 8 CFU / mL soaked in normal marsh shrimp could not replicate the disease. The bacteria in the diseased shrimp were soaked in normal shrimp at a concentration of 1: 50 and 1: 250 for 7 days After the onset of disease, and the typical symptoms of muscle white turbidity, chloroform treatment can not destroy the virus infectivity; Slim shrimp ultra-thin section electron microscopy found muscle tissue exists in the size of 2 1- 2 3nm the size of the virus, After treatment with chloroform, PEG precipitation, phosphotungstic acid negative staining can be seen a large number of 2-2-4nm size spherical virus particles. The above results indicate that M. turcicola is a disease caused by a virus.