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目的研究青蒿琥酯抑制胃腺癌细胞生长及调控细胞周期作用。方法利用流式细胞术方法检测不同胃病变组织中细胞周期素D1(CyclinD1)蛋白表达水平。不同浓度(0、30、60、120μmol/L)青蒿琥酯(artesunate,Art)干预胃腺癌SGC-7901细胞24h,0μmol/L Art采用生理盐水代替药物作为对照组,流式细胞术检测细胞周期及CyclinD1蛋白表达水平,并分析SGC-7901细胞中CyclinD1蛋白表达水平与细胞周期G0/1期相关性。结果胃腺癌组织中CyclinD1蛋白表达水平显著高于正常胃组织中的表达水平,P<0.01。不同浓度Art组与对照组相比,SGC-7901细胞周期G0/1期显著增多,P<0.01,且具有Art剂量依赖性,而CyclinD1蛋白表达水平显著降低,P<0.01,具有Art剂量依赖性;CyclinD1蛋白表达与细胞周期G0/1期呈显著负相关,P<0.01。结论 CyclinD1在胃腺癌组织中的高表达导致了细胞周期紊乱,从而参与了胃腺癌发生及发展,Art诱导胃腺癌SGC-7901细胞周期阻滞于G0/1期,与下调细胞中CyclinD1蛋白表达有关,从而起到抗胃腺癌作用。
Objective To study the inhibitory effect of artesunate on the growth of gastric adenocarcinoma cells and the regulation of cell cycle. Methods Flow cytometry was used to detect the expression of CyclinD1 in different gastric tissues. Artesunate (Art) intervention with different concentrations (0, 30, 60 and 120μmol / L) of gastric adenocarcinoma SGC-7901 cells 24h, 0μmol / L Art with saline instead of drugs as a control group, flow cytometry cells Cycle and CyclinD1 protein expression levels in SGC-7901 cells and analyze the relationship between the expression level of CyclinD1 and cell cycle G0 / 1 in SGC-7901 cells. Results The expression of CyclinD1 in gastric adenocarcinoma was significantly higher than that in normal gastric tissues (P <0.01). Compared with the control group, the G0 / 1 phase of SGC-7901 cells was significantly increased at different concentrations of Art group (P <0.01), Art (2) was dose-dependent and the expression of CyclinD1 protein was significantly decreased ; CyclinD1 protein expression and cell cycle G0 / 1 was significantly negative correlation (P <0.01). Conclusion The high expression of CyclinD1 in gastric adenocarcinoma leads to cell cycle disorder, which is involved in the occurrence and development of gastric adenocarcinoma. Art induced apoptosis of gastric adenocarcinoma cell line SGC-7901 in G0 / 1 phase, which is associated with the downregulation of CyclinD1 protein , Which play a role in anti-gastric cancer.