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目的 研究癌性体液脱落细胞的端粒酶活性及在良恶性体液的鉴别诊断中的应用价值。方法 应用 PCR- ELISA方法检测体液脱落细胞中端粒酶活性 ,并将检测结果与细胞学检查进行比较。结果 癌性胸、腹水端粒酶阳性率为 61 .1 1 % (33/54) ;其中 ,肺癌胸水脱落细胞端粒酶阳性率为 70 % (2 1 /30 ) ,而形态学阳性率为 53.33% (1 6/30 )。膀胱癌尿液端粒酶阳性率为 83.64%(46/55) ,而形态学阳性率为 52 .72 % (2 9/55)。结论 端粒酶活性检测在癌性体液的诊断和鉴别诊断方面有重要的应用价值。
Objective To study the telomerase activity of exfoliative cells in cancerous body fluids and its application in the differential diagnosis of benign and malignant body fluids. Methods The telomerase activity in exfoliated cells was detected by PCR-ELISA and the results were compared with cytological tests. Results The positive rate of telomerase in cancerous thoracic and ascites was 61.11% (33/54). Among them, positive rate of telomerase in pleural effusion exfoliated cells was 70% (2 1 /30), and the positive rate of morphology was. 53.33% (1 6/30). The positive rate of telomerase in bladder cancer was 83.64% (46/55), and the positive rate of morphology was 52.72% (29/55). Conclusion The detection of telomerase activity has important application value in the diagnosis and differential diagnosis of cancerous body fluids.