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本文将采用木瓜蛋白酶水解和ＳＰＡ－Ｓｅｐｈａｒｏｓｅ４Ｂ柱亲和层析等手段获得的人ＩｇＧＦｃ和Ｆａｂ，以抗人ＩｇＧＦｃ和抗人ＩｇＧＦａｂ单抗为参比品（Ｓｉｇｍａ）．鉴定了细胞库中抗人ＩｇＧ系列的部分细胞林，得到分泌特异性抗人ＩｇＧＦｅ和抗人ＩｇＧＦａｂ单抗的细胞各一株。在此基础上。应用抗人ＩｇＧＦｃ及抗人ＩｇＧＦａｂ单抗分别制备了Ｓｅｐｈａｒｏｓｅ４Ｂ亲和层析柱，纯化了酶解的人ＩｇＧＦｃ和Ｆａｂ。经ＥＬＩＳＡ法鉴定，相互间无交叉反应。同时用此方法还制备了人抗ＨＢｅＦａｂ，并将此Ｆａｂ标记过氧化物酶。配制ＨＢｅＥＬＩＳＡ诊断盒，证明其生物学活性未受影响，而且消除了类风湿因子引起的ＨＢｅＡｇ假阳性现象。
In this paper, human IgGFc and Fab obtained by papain hydrolysis and SPA-Sepharose 4B column affinity chromatography, anti-human IgG and anti-human IgGFab monoclonal antibody as a reference (Sigma). A part of the cell line of anti-human IgG series in the cell bank was identified, and one strain secreting specific anti-human IgGFe and anti-human IgGFab monoclonal antibodies was obtained. on the basis of. Sepharose 4B affinity chromatography was prepared using anti-human IgGFc and anti-human IgGFab monoclonal antibody respectively, and purified human IgGFc and Fab were purified. Identified by ELISA, no cross-reaction between each other. In the meantime, human anti-HBeFab was also prepared by this method, and this Fab was labeled with peroxidase. The HBeELISA diagnostic kit was formulated to demonstrate that its biological activity was unaffected and that the HBeAg false positives caused by rheumatoid factor were eliminated.