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[目的]利用生物标志物作为农药毒性评价的研究受到日益广泛的重视,为了研究苯醚甲环唑和丙环唑对水生生物的毒性效应,寻找生物标志物。[方法]以48 h-EC50质量浓度0.52 mg/L处理48 h测定了大型溞体内GST酶活力与时间的效应关系以及不同浓度药剂处理48 h后GST酶活力与剂量的效应关系。[结果]不同时间处理时,随苯醚甲环唑和丙环唑处理时间的延长呈GST酶活性被诱导的幅度提高,处理48 h后酶活力达到最高值。不同质量浓度处理时,随苯醚甲环唑质量浓度的提高,GST酶活力诱导率增大,1.00 mg/L时诱导率最大,达到324.84%。[结论]试验结果表明GST酶可以作为苯醚甲环唑和丙环唑对大型溞毒性影响的生物标志物。
[Objective] The research of using biomarkers as the evaluation of pesticide toxicity has been paid more and more attention. In order to study the toxic effects of difenoconazole and propiconazole on aquatic organisms, we searched for biomarkers. [Method] The effect of GST enzyme activity on the time and the effect of GST enzyme activity on the dosage of 48 h-EC50 for 48 h were studied. [Result] The results showed that the GST enzyme activity was increased with the time of difenoconazole and propiconazole being treated at different time, and reached the maximum after 48 h of treatment. With different concentrations of difenoconazole, the induction rate of GST enzyme activity increased with the increase of difenoconazole mass concentration, and the highest induction rate was reached at 1.00 mg / L, reaching 324.84%. [Conclusion] The results of the experiment indicated that GST enzyme could be used as a biomarker of difenoconazole induced toxicity of difenoconazole and propiconazole.