目的检测六味地黄汤经光合细菌代谢后β-胡萝卜素含量的变化。方法以β-胡萝卜素为检测指标,采用紫外分光光度法测定光合细菌中β-胡萝卜素的含量,紫外检测波长为451nm。结果光合细菌代谢六味地黄汤后β-胡萝卜素含量发生变化;β-胡萝卜素平均回收率为99.69%,RSD=1.04%(n=6)。结论该方法简单易行,灵敏度高,准确度高,可用于光合细菌中β-胡萝卜素含量的测定。 Objective To detect the changes of β-carotene content of Liuweidihuangtang metabolized by photosynthetic bacteria. Methods The content of β-carotene in photosynthetic bacteria was determined by UV-spectrophotometry with β-carotene as the detection index. The UV detection wavelength was 451 nm. Results After the photosynthetic bacteria metabolized, the content of β-carotene changed. The average recovery rate of β-carotene was 99.69%, RSD was 1.04% (n = 6). Conclusion The method is simple, sensitive, accurate and can be used for the determination of β-carotene content in photosynthetic bacteria.