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本研究观察了顺铂（ＣＤＤＰ）对猪肾近曲小管上皮细胞（ＬＬＣ－ｐＫ＿１）增殖与ＤＮＡ合成代谢的影响以及亚硒酸钠与硒代蛋氨酸的保护作用。结果表明，ＣＤＤＰ对ＬＬＣ－ｐｋ＿１细胞具有明显的毒性作用，在５～１５０μｍｏｌ／Ｌ范围内，ＣＤＤＰ与ＬＬＣ－ｐＫ＿１细胞作用１２ｈ，对细胞增殖已呈明显的抑制作用，其ＩＣ＿（５０）为１０９．５μｍｏｌ／Ｌ；作用２４ｈ时，其抑制效应更加明显，并存在剂量效应关系。ＬＬＣ－ｐｋ＿１细胞ＤＮＡ合成代谢对ＣＤＤＰ更为敏感，作用３ｈ，ＤＮＡ合成就受到明显的抑制，其ＩＣ＿（５０）为１２０．５μｍｏｌ／Ｌ。ＣＤＤＰ与ＬＩＣ－Ｐｋ＿１细胞作用３ｈ，对ＲＮＡ及蛋白质合成也具有明显的抑制作用。Ｎａ２ＳｅＯ３与Ｄ，Ｌ－ＳｅＭｅｔ两种不同硒化合物，当硒与ＣＤＤＰ的浓度比为１：１０时，Ｎａ２ＳｅＯ３未显示对ＣＤＤＰ的ＤＮＡ合成抑制作用有何保护作用，而Ｄ，Ｌ－ＳｅＭｅｔ则在一定程度上能减轻ＣＤＤＰ对ＬＬＣ－ｐｋ＿１细胞ＤＮＡ合成的抑制作用，但Ｄ，Ｌ－ＳｅＭｅｔ提前或与ＣＤＤＰ同时加入，其保护作用没有明显差异。
This study investigated the effects of cisplatin (CDDP) on the proliferation and DNA synthesis and metabolism of porcine renal proximal tubule epithelial cells (LLC-pK_1) as well as the protective effects of sodium selenite and selenomethionine. The results showed that CDDP had a significant toxic effect on LLC-pk_1 cells. CDDP and LLC-pK_1 cells treated with LLC-pK_1 at a dose of 5 ~ 150μmol / L for 12h had a significant inhibitory effect on cell proliferation, with IC 50 of 109 .5μmol / L; 24h, the inhibitory effect was more obvious, and there is dose-response relationship. The DNA synthesis and metabolism of LLC-pk_1 cells were more sensitive to CDDP, and the DNA synthesis was significantly inhibited after 3 hours, with an IC 50 value of 120.5 μmol / L. CDDP and LIC-Pk_1 cells 3h, RNA and protein synthesis also had significant inhibitory effect. Na2SeO3 and D, L-SeMet two different selenium compounds, when the concentration of selenium and CDDP ratio of 1:10, Na2SeO3 did not show any protective effect of CDDP DNA synthesis inhibition, and D, L-SeMet in a certain The inhibitory effect of CDDP on DNA synthesis in LLC-pk_1 cells was alleviated to a certain extent. However, there was no significant difference in the protective effect if D, L-SeMet was added in advance or simultaneously with CDDP.