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目的 评估腹壁撤回反射(AWR)评分用于评价大鼠结直肠扩张内脏痛动物模型的行为反应及可靠性。方法 大鼠经肛门插入扩张气囊,注气扩张结直肠建立急性内脏痛动物模型。采用腹壁撤回反射评分评估大鼠受伤害性刺激而出现的行为反应强度,并测定 AWR 3分的最小压力值(痛阈)。结果 大鼠结直肠扩张后100％出现腹壁撤回反射,扩张压力越高,其评分越高,扩张压力 15、25、40、60 mm Hg(1mm Hg=0.133 kPa)产生的 AWR评分相互比较差异均有显著性(P<0.05);首次扩张的痛阈较高,3次扩张后稳定,其平均痛阈为(27.68±2.59)mm Hg,5、10 d后重复扩张痛阈无明显变化;吗啡皮下注射呈剂量依赖提高大鼠结直肠扩张的痛阈,其作用可被纳洛酮完全拮抗。结论 腹壁撤回反射评分可评价大鼠结直肠扩张的刺激强度,其中 AWR 3分指标明确,测定的痛阈值稳定;该模型及其行为学评分具有无创伤、稳定性好、重复性强、可定量的特点,可用于内脏痛及镇痛药的有关研究。
Objective To assess the abdominal wall retracement reflectance (AWR) score to evaluate the behavioral response and reliability of rat model of colorectal distension and visceral pain. Methods After the anus was inserted into the dilated balloon, the animal models of acute visceral pain were established by injecting gas into the dilated colorectal. The abdomen reflex score was used to assess the behavioral response to noxious stimulation in rats and the minimum pressure (pain threshold) for AWR score 3 was determined. Results The collaterals were retrograde and the abdomen reflex was observed in 100% of the rats. The higher the dilatation pressure, the higher the score was. The AWR scores of 15,25,40 and 60 mm Hg (1mm Hg = 0.133 kPa) (P <0.05). The pain threshold of the first expansion was high, and after 3 times of expansion, the average pain threshold was (27.68 ± 2.59) mm Hg. There was no significant change of pain threshold after 5 and 10 days. Morphine Subcutaneous injection in a dose-dependent manner to increase the pain threshold of colorectal distension in rats, its role can be completely antagonized by naloxone. Conclusion Abdominal wall reflex score can evaluate the stimulating intensity of colorectal distention in rats. The AWR score of 3 points is clear and the pain threshold value is stable. The model and its behavioral score are noninvasive, good stability, strong repeatability and can be quantified The characteristics of visceral pain and analgesics can be used for the relevant research.