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目的为揭示环境因素及维生素D受体(VDR)基因SNP rs2222570单核苷酸多态性与河南地区结核病发病相关性。方法本研究采样病例对照研究设计,于2016年2月至10期间在郑州市结防所收集涂阳肺结核病人140例,并以年龄性别匹配取同时期140例健康体检人群作为对照。对抽取人群进行问卷调查和静脉血采集,并用聚合酶链反应结合测序结果分析对维生素D受体基因SNP rs2222570多态性。进行单因素卡方检验和多因素非条件Logistics回归分析。结果多因素logistics回归表明结核病接触史(OR=21.38 95%CI=6.4~36.08),吸烟(OR=4.27 95%CI=2.34~9.52)、粉尘接触史(OR=2.84 95%CI=1.95~6.97)、卡介苗接种(OR=0.47 95%CI=0.007~0.33)、睡眠时间(OR=0.14 95%CI=0.02~0.76)和维生素D受体基因SNP rs2222570基因型C/C(OR=0.72 95%CI=0.55~0.93)与结核发病相关性有统计学意义。VDR基因C/C基因型作为遗传因素与环境因素中卡介苗接种、吸烟和结核病人接触史均存在交互作用。结论 VDR基因SNP rs2222570多态性与传统环境因素在结核病发生中存在一定交互作用。该研究结果为了解结核病发生、发展机制,预防控制该病的发生和流行提供了科学依据。
Objective To reveal the environmental factors and vitamin D receptor (VDR) gene SNP rs2222570 single nucleotide polymorphisms and the incidence of tuberculosis in Henan Province. Methods In this study, we sampled 140 cases of smear positive pulmonary tuberculosis from February to October 2016 in Zhengzhou City, and compared them with 140 healthy people in the same period by gender. Questionnaires and venous blood samples were collected from the extracted population, and the SNP rs2222570 polymorphism of vitamin D receptor gene was analyzed by polymerase chain reaction combined with sequencing results. Univariate chi-square test and multi-factor non-conditional Logistics regression analysis. Results The logistic regression showed that the contact history of tuberculosis (OR = 21.38 95% CI 6.4-36.08), smoking (OR 4.27 95% CI 2.34-9.52) and dust exposure history (OR = 2.84 95% CI 1.95 6.97 BCG vaccination (OR = 0.47 95% CI = 0.007-0.33), sleep time (OR = 0.14 95% CI = 0.02-0.76) and vitamin D receptor gene SNP rs2222570 genotype C / C CI = 0.55 ~ 0.93) and the incidence of tuberculosis was statistically significant. VDR gene C / C genotype as a genetic factor and environmental factors in the BCG vaccination, smoking and TB exposure history there is interaction. Conclusion The SNP rs2222570 polymorphism of VDR gene interacts with the traditional environmental factors in the pathogenesis of tuberculosis. The results of this study provide a scientific basis for understanding the pathogenesis and prevalence of tuberculosis, development mechanism and prevention and control of the disease.