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以二棱大麦品种间杂交种F_1(2n=2x=14)为母本,以苏联球茎大麦(2n=4x=28)为父本,进行种间杂交,授粉一天后。在母本柱头滴赤霉酸(GA_3),并结合离体培养杂种幼胚,获得较多二棱大麦与球茎大麦的种间杂种,根据杂种植株形态和染色体组成,可分成三种类型,其中只有约5%的植株的染色体加倍成功,为大麦型(2n=14);其余95%植株的形态表现为偏大麦型与偏球茎型的两种中间型,且均为具球茎的多年生习性;其染色体数在15—21之间:目交不育,用父母本回交亦未结实。
Two-barley crossbred F_1 (2n = 2x = 14) was used as the female parent, and the tuberous barley (2n = 4x = 28) was used as the male parent for interspecific hybridization. In the female parent stigma GA3, combined with in vitro culture of hybrid embryos, access to more barley and bulb barley interspecific hybrids, hybrid plants according to morphology and chromosome composition, can be divided into three types, including Only about 5% of the plant chromosomes were doubled, which was barley (2n = 14). The remaining 95% of the plants showed two types of midspecies: barley and perennial. The chromosome number between 15-21: eye contact infertility, with the parents of the back did not fruition.