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建立ＰＣＲ ＲＦＬＰ ＳＳＣＰ及测序方法 ,用于分析霍乱弧菌肠毒素ＡＢ(ｃｔｘ ＡＢ)基因多态性。针对ｃｔｘ ＡＢ基因设计引物 ,扩增片段为 5 2 8ｂｐ ,包括ｃｔｘ Ｂ编码基因375ｂｐ ,引物序列为 5′ ＧＧＴＧＴＡＡＡＡＴＴＣＣＴＴＧＡＣＧ 3′和 5′ ＧＧＴＴＣＧＡＴＧ
To establish PCR RFLP SSCP and sequencing method for the analysis of Vibrio cholerae enterotoxin AB (ctx AB) gene polymorphism. Primers were designed for the ctx AB gene. The amplified fragment was 528bp, including 375bp of ctx B coding gene and 5 ’GGTGTAAAATTCCTTGACG 3’ and 5 ’GGTTCGATG