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通过石油醚、乙酸乙酯、正丁醇萃取,利用硅胶柱色谱、凝胶Sephadex LH-20、ODS柱色谱等方法,对鲜黄独块茎Dioscorea bulbifera的95%乙醇提取物化学成分进行分离纯化,并运用波谱学技术手段对分离得到的单体化合物进行结构鉴定。从鲜黄独药材分离鉴定了18个化合物,依次为6-羟基-2,10,10-三甲氧基-9-蒽酮(1)、薯蓣皂苷元(2)、豆甾醇(3)、3,7-二甲氧基-5,3,4-三羟基黄酮(4)、2,7-二羟基-3,4-二甲氧基菲(5)、3,7-二羟基-2,4-二甲氧基菲(6)、2,7-二羟基-4-甲氧基菲(7)、2,7-二羟基-3,4-二甲氧基-9,10-二氢菲(8)、壬二酸(9)、8-表黄独素E(10)、1,7-双-(4-羟基苯基)-4E,6E-庚二烯-3-酮(11)、黄独素B(12)、二十五烷酸-α-单甘油酯(13)、2,7-二羟基-4-甲氧基-9,10-二氢菲(14)、1,7-双-(4-羟基苯基)-1E,4E,6E-庚三烯-3-酮(15)、6-乙氧基-1H-嘧啶-2,4-二酮(16)、3,5,4’-三羟基联苄(17)、黄独素F(18)。其中化合物1为新化合物,化合物7,9,13,16为首次从该属植物中分离得到。
The chemical constituents of 95% ethanol extract of Dioscorea bulbifera were isolated and purified by petroleum ether, ethyl acetate and n-butanol using silica gel column chromatography, gel Sephadex LH-20 and ODS column chromatography. The structure of the isolated monomer compounds was identified by spectroscopic techniques. Eighteen compounds were isolated and identified from fresh yellow medicinal herbs, which were 6-hydroxy-2,10,10-trimethoxy-9-anthrone (1), diosgenin (2), stigmasterol (4), 2,7-dihydroxy-3,4-dimethoxy phenanthrene (5), 3,7-dihydroxy-2, Dimethoxyphenanthrene (6), 2,7-dihydroxy-4-methoxyphenanthrene (7), 2,7-dihydroxy-3,4-dimethoxy-9,10-dihydro (11), phenanthrene (8), azelaic acid (9), 8-epoxyphenol E (10), 1,7- bis- (4- hydroxyphenyl) -4E, 6E-heptadin- (12), pentacosanoic acid-alpha-monoglyceride (13), 2,7-dihydroxy-4-methoxy-9,10-dihydrophenanthrene (14), 1,7-bis - (4-hydroxyphenyl) -1E, 4E, 6E-heptatrien-3-one (15) 4’-trihydroxybibenzyl (17), Huang prime F (18). Among them, compound 1 was a new compound, and compounds 7, 9, 13 and 16 were isolated from this genus for the first time.