大鼠正常肝和一系列有不同生长速度与分化程度的移植性肝癌,经制成匀浆、并超速离心分离后制得上清液,通过DBAE-纤维素柱层析,籍氯离子梯度洗脱,可以看到丙酮酸盐激酶(简称 PK,下同)有三种同功酶型式。其中 PKⅠ是正常大鼠肝的主要酶型,占总酶活力的90%,达57单位/克组织,它在0.10～0.15MG1~-梯度中洗下;PKⅡ是心脏和骨髂肌所 Rat normal liver and a series of different growth rates and degree of differentiation of transplanted liver cancer, made of homogenate and ultracentrifugation obtained supernatant, by DBAE-cellulose column chromatography, washed by chloride ion gradient Off, you can see pyruvate kinase (hereinafter referred to PK, the same below) have three isozyme type. Among them PKI is the main type of enzyme in normal rat liver, accounting for 90% of the total enzyme activity, up to 57 units / gram of tissue, which elutes in a gradient of 0.10-0.15 MG1; PKII is the heart and bone iliac muscle