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目的探讨早期胰岛素强化治疗对非肥胖糖尿病(NOD)小鼠T细胞亚群的免疫调节机制。方法 12~14周龄NOD小鼠30只随机分为早期强化治疗组(EIT组)、早期常规治疗组(ECT组)、晚期强化治疗组(LIT组)、晚期常规治疗组(LCT组)、糖尿病对照组(DM组),另选取同周龄未发病雌性NOD小鼠6只为正常对照组(NC组)。比较EIT组与其他各组体质量、24h动态血糖及干预治疗后脾脏和胸腺T细胞亚群的变化情况。结果 EIT组治疗后体质量高于LIT组、LCT组和DM组(P<0.001),接近NC组(P>0.05),24h动态血糖平均值基本维持在(5.758±1.515)mmol/L,低于LIT组、LCT组和DM组(P<0.001),高于NC组(P<0.05),诱导脾脏中CD4+、CD3+T细胞百分比低于LCT组和DM组(P<0.001),并使CD4+/CD8+亚群比值低于LCT组和DM组(P<0.001),同时诱导胸腺CD4+CD8-单阳性细胞百分比低于LIT组、LCT组和DM组(P<0.001),CD4+CD8+双阳性细胞百分比与LIT组、LCT组和DM组比较差异均有统计学意义(P<0.001)。结论早期胰岛素强化治疗有效控制NOD小鼠血糖和体质量在正常水平;早期强化治疗可以通过调节宿主脾脏和胸腺淋巴细胞亚群的变化调节自身免疫反应。
Objective To investigate the immunoregulatory mechanism of early intensive insulin therapy on T cell subsets in non-obese diabetic (NOD) mice. Methods Thirty NOD mice aged 12 to 14 weeks were randomly divided into three groups: EIT group, ECT group, LIT group, LCT group, In the diabetic control group (DM group), 6 female NOD mice of the same age were also selected as the normal control group (NC group). The EIT group and other groups body mass, 24h dynamic blood glucose and intervention in the spleen and thymus T cell subsets changes. Results After treatment, the body weight of EIT group was significantly higher than that of LIT group, LCT group and DM group (P <0.001), and close to the NC group (P> 0.05). The average 24h dynamic blood glucose level remained at (5.758 ± 1.515) mmol / L The percentage of CD4 + and CD3 + T cells in spleen was lower than that in LCT group and DM group (P <0.001) in LIT group, LCT group and DM group (P <0.001) The percentage of CD4 + / CD8 + subsets in LCT group and DM group was lower than that in LCT group and DM group (P <0.001), while the percentage of CD4 + CD8- single positive cells in thymus was lower than that in LIT group, LCT group and DM group The percentage of sex cells was significantly different from LIT group, LCT group and DM group (P <0.001). Conclusion Early intensive insulin therapy can effectively control blood glucose and body weight of NOD mice at normal level. Early intensive treatment can regulate autoimmune reaction by regulating the changes of lymphocyte subsets in the spleen and thymus of the host.