Objective: To summarize and critically assess the inhibitory effects of Chinese herbal medicine (CHM) on tumor volume and tumor weight for the treatment of osteosarcoma (OS) in mouse models. Methods:PubMed, Embase, Web of Science, China Knowledge Resource Integrated Database (CNKI), Wanfang Database, VIP Database, and Chinese BioMedical (CBM) were searched since their inception dates to March10, 2016. Two reviewers independently selected the controlled studies estimating effects of CHM on mouse OS by administration in vivo. A pair-wise meta-analysis was performed. Twenty-five studies with adequate randomization were included in the systematic review. Results: CHM may signi?cantly inhibit OS growth in mice, as assessed using the tumor weight [20 studies, n=443; 290 for CHM and 153 for the control; pooled mean difference (MD) =–2.90; 95％ confidence interval (CI): –3.50 to –2.31; P<0.01], tumor volume (16 studies, n=382; 257 for CHM and 125 for the control; pooled MD =–2.57; 95％ CI: –3.33 to –1.80; P<0.01) and tumor growth inhibition rate. Conclusion: CHM could significantly inhibit the growth of OS in mouse models, which might be supportive for the design of preclinical and clinical trials in future.