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目的 :应用流式细胞术AV/PI法研究胃癌组织的早期凋亡情况。方法 :手术标本分离单细胞悬液 ,经AV -FITC与PI标记后流式细胞仪测定凋亡率。结果 :分离的单细胞经标记检测后可明确区分凋亡细胞、坏死细胞与活细胞的比例 ;其比例关系在肿瘤组织与对照组之间有显著性差异。结论 :AV/PI双参数法流式细胞仪检测可用于胃癌组织的早期凋亡的检测 ,为胃癌的临床研究提供可行方法
Objective : To investigate the early apoptosis of gastric cancer by flow cytometry AV/PI method. METHODS: Single cell suspensions were isolated from surgical specimens and the apoptosis rate was determined by flow cytometry after AV-FITC and PI labeling. Results : The ratio of apoptotic cells and necrotic cells to viable cells could be clearly distinguished by the single cell isolation test. There was a significant difference in the ratio between the tumor tissue and the control group. Conclusion : The AV/PI dual-parameter flow cytometry can be used to detect the early apoptosis of gastric cancer and provide a feasible method for the clinical research of gastric cancer.