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在中华医学会检验学分会主任委员丛玉隆教授组织召开的“四省市SARS实验诊断学术研讨会”、“北京SARS诊断管理和学术研讨会”及“全国五省市SARS实验诊断学术研讨会”上,检验学界的专家们专门就目前SARS实验室诊断方面存在的问题进行了探讨、分析和总结。 专家们一致认为,对SARS的诊断至今仍以临床为主,但其确诊或疑似标准并不完善,急需用实验诊断的方法发现病原的证据,以进行SARS的确诊和鉴别诊断。目前,确定诊断的方法主要有病毒培养、免疫学检测及分子诊断法。病毒培养通常对实验室要求很高,检验医学科难以进行;分子诊断法目前还很不完善;免疫学测定有免疫荧光实验(IFA)和酶联免疫吸附实验(ELISA)。IFA测定特异性强,但灵敏度较低并需要有固定的SARS病毒颗粒,更适合检验医学科应用的是ELISA法。然而遗憾的是,经过临床验证,ELISA的检测结果不尽如人意。问题出在哪里?如何解决?
In the “Symposium on Experimental Diagnosis of SARS in Four Provinces and Cities” organized by Professor Cong Yulun, Chairman of the Examination Branch of Chinese Medical Association, “Beijing SARS Diagnostic Management and Symposium” and “Symposium on SARS Experimental Diagnosis in Five Provinces and Cities” , Experts in the field of testing have specifically discussed, analyzed and summarized the problems existing in SARS laboratory diagnosis. Experts agreed that the diagnosis of SARS is still clinically based, but its diagnosis or suspected standard is not perfect, the urgent need to use experimental diagnosis of pathogen evidence to confirm the diagnosis and differential diagnosis of SARS. Currently, the main methods to determine the diagnosis of virus culture, immunological detection and molecular diagnosis. Virus culture usually requires very high laboratories, laboratory medicine is difficult to carry out; molecular diagnostic method is still not perfect; immunological assay IFA and ELISA. IFA assay specificity, but less sensitive and requires a fixed SARS virus particles, more suitable for testing medical applications is the ELISA method. However, unfortunately, after clinical validation, ELISA test results are not satisfactory. Where is the problem? How to solve?