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目的 :用电穿击化疗对异位移植裸小鼠人结直肠肿瘤肝转移模型进行治疗 ,探讨其疗效和机制。方法 :将 4 0只皮下人结肠癌LoVo细胞系肿瘤的裸鼠随机分成 1 0区组 ,每区组 4只 ,随后将每区组的小鼠分至电穿击化疗组、化疗组、电击组、阴性对照组 ,并给予相应的治疗。 1w后处死小鼠 ,观察瘤重及病理改变情况。结果 :电穿击化疗组小鼠的肿瘤显著减小 ,其中有一只小鼠的肿瘤完全消失 ,平均瘤重为 1 8.3mg ,与其余各组平均瘤重 (化疗组 36 .9mg、电击组 37.9mg、阴性对照组 4 2 .7mg)相比在统计学上差异有显著性 ,P<0 .0 1。光镜下可见电穿击化疗组的肿瘤组织有大量片状坏死、炎性细胞浸润、血管的扭曲及内皮的脱落和坏死 ,而化疗组和电击组则仅见少量炎性细胞的浸润和单个细胞的坏死。结论 :电穿击化疗可以显著增强肿瘤对化疗的敏感性 ,为人结直肠肿瘤肝转移的治疗提供了一种新的途径
OBJECTIVE: To study the efficacy and mechanism of electroporation chemotherapy for human colorectal liver metastasis model of xenografted nude mice. Methods: Twenty nude mice bearing tumors of LoVo human colon cancer cell line LoVo were randomly divided into 10 groups, 4 in each group. The mice in each group were then divided into electrochemotherapy group, chemotherapy group, electric shock Group, negative control group, and given the appropriate treatment. After 1 w mice were sacrificed to observe the tumor weight and pathological changes. Results: The tumor of electroporation chemotherapy group was significantly reduced, and the tumor of one mouse completely disappeared. The average tumor weight was 1 8.3mg, which was significantly higher than that of other groups (36.9mg in chemotherapy group, 37.9 in shock group mg, negative control group 4 2 .7mg) compared to statistically significant differences, P <0. Light microscopic examination showed that there were a large number of tumor necrosis, infiltration of inflammatory cells, vascular distortions, and shedding and necrosis of endothelial cells in the tumor tissue of the electro-puncture chemotherapy group, while only a few inflammatory cell infiltration and single cells were seen in the chemotherapy and shock groups The necrosis. Conclusion: Electro-shock chemotherapy can significantly enhance the sensitivity of the tumor to chemotherapy and provide a new approach for the treatment of colorectal cancer with liver metastasis