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目的:建立HPLC法测定复方防风胶囊中升麻素苷、5-0-甲基维斯阿米醇苷含量的方法。方法:采用Waters svmmetry-C_(18)色谱柱(250 mm×4.6 mm,5μm),流动相为甲醇-0.1%冰醋酸,梯度洗脱,检测波长254 nm,柱温为30℃。结果:升麻素苷在10～160 mg·L~(-1)范围内有良好线性关系(r=0.9997),平均回收率为100.10%(RSD=1.89%)。5-O-甲基维斯阿米醇苷在10～80 mg·L~(-1)范围内有良好线性关系(r=0.9999),平均回收率为100.29%(RSD=1.61%)。结论:该方法灵敏、准确、可靠,可作为复方防风胶囊的质量控制。
OBJECTIVE: To establish a HPLC method for the determination of cortisol and 5-O-methyl-Vaisalanthin in Compound Fangfengfeng Capsules. Methods: Waters svmmetry-C 18 column (250 mm × 4.6 mm, 5 μm) was used. The mobile phase consisted of methanol-0.1% glacial acetic acid with gradient elution. The detection wavelength was 254 nm and the column temperature was 30 ℃. Results: Cinarin had a good linear relationship (r = 0.9997) in the range of 10 ~ 160 mg · L -1 with an average recovery of 100.10% (RSD = 1.89%). There was a good linear relationship between 5-O-methylWestoside and 10 ~ 80 mg · L -1 (r = 0.9999), with an average recovery of 100.29% (RSD = 1.61%). Conclusion: The method is sensitive, accurate and reliable and can be used as the quality control of compound Fangfeng capsule.