组织在固定过程中,由于甲醛、加热、有机溶剂的处理,使抗原发生交联、变性等,致使在免疫组化染色过程中无法与抗体结合。但这一变化是可逆的,可通过抗原修复重新暴露抗原决定簇,增加抗原抗体结合的机会,从而提高免疫组化染色效果[1,2]。本工作从影响蛋白构象的温度、pH、表面 Tissues in the fixed process, due to formaldehyde, heating, organic solvent treatment, the antigen cross-linking, degeneration, resulting in the process of immunohistochemical staining with antibodies. However, this change is reversible, antigen retrieval can be re-exposed antigenic determinants, increased antigen-antibody binding opportunities, thereby enhancing the immunohistochemical staining [1,2]. This work from the impact of protein conformation temperature, pH, surface