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以锦带花属的红王子锦带、花叶锦带和四季锦带3个优良的锦带花栽培品种当年生枝条为试材,通过人工冰冻方法,测定了模拟低温0(CK)、-10、-20、-30、-40℃条件下3个锦带花品种离体枝条的K+相对渗出率和相对电导率,同时利用Logistic方程拟合K+相对渗出率及相对电导率与人工模拟低温变化关系,推算出3个品种的低温半致死温度(LT50);并对3个品种离体枝条的自由水含量、束缚水含量以及二者比值的变化进行了比较。结果表明:在模拟低温条件下,3个品种离体枝条的K+相对渗出率和相对电导率均随温度降低逐渐增大,且总体上与CK有显著差异;对K+相对渗出率和相对电导率与温度进行Logistic方程拟合推算出花叶锦带、红王子锦带、四季锦带的半致死温度分别为-35.60、-32.21、-31.50℃和-34.88、-31.14、-28.44℃;随着胁迫温度的降低,3个品种的束缚水含量均高于自由水含量,其中束缚水和自由水的比值排序为花叶锦带>红王子锦带>四季锦带。综合比较,3个锦带花品种抗寒性强弱顺序为花叶锦带>红王子锦带>四季锦带。
The results showed that simulated low temperature 0 (CK), simulated low temperature (CK), low temperature stress K + relative exudation rate and relative conductivity of detached shoots of three strains of Gentiana straminea at 10, -20, -30 and -40 ℃, respectively. Logistic equation was also used to fit K + relative exudation rate and relative conductivity with artificial The LT50 of three cultivars was calculated. The free water content, irreducible water content and the ratio of the two were compared. The results showed that K + relative exudation rate and relative conductivity of detached shoots of three cultivars increased gradually with the decrease of temperature under the simulated low temperature conditions, and there was a significant difference with CK in general. The relative exudation rates of K + The semi-lethal temperature of the mosaic zone and the red princely zonal zone was -35.60, -32.21, -31.50 ℃ and -34.88, -31.14 and -28.44 ℃ respectively by logistic equation fitting of conductivity and temperature. With the decrease of stress temperature, the irreducible water content of the three cultivars was higher than that of free water, and the ratio of irreducible water to free water was ranked as Moutan> red prince>> seasons. Taken together, the order of cold resistance of three Jinhua flowers is Mosaic belt> Red prince Jin belt> Four seasons Jin belt.