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目的考察阿莫西林干预对婴儿优势菌双歧杆菌及乳杆菌在无菌小鼠体内定植的影响。方法 1日龄Balb/c无菌乳鼠接种婴儿粪便悬液。饲养至7~21日龄灌胃阿莫西林(100mg/kg),对照组在同日龄给予等体积的生理盐水。利用qRT-PCR检测小鼠粪便中双歧杆菌、乳杆菌的含量。结果阿莫西林处理可显著降低乳杆菌(P<0.05)、双歧杆菌(P<0.05)在无菌小鼠体内定植数量,但停药后饲养至成年(53日龄)二者定植数量与对照组小鼠比较差异无统计学意义(P>0.05)。结论哺乳期阿莫西林干预会导致乳杆菌、双歧杆菌在小鼠体内定植量下降,但停药后小鼠饲养至成年二者可达到正常定植量,婴儿菌群定植小鼠模型可以模拟与现有动物模型一致的阿莫西林对双歧杆菌、乳杆菌定植的影响规律。
Objective To investigate the effect of amoxicillin on colonization of infant infant Bifidobacterium and Lactobacillus in germ-free mice. Methods One-day-old Balb / c gerbils were inoculated with infant droppings. The animals were housed to amoxicillin (100 mg / kg) at 7 to 21 days of age, and the control group was given the same volume of normal saline at the same age. QRT-PCR was used to detect bifidobacteria and lactobacilli in feces of mice. Results Amoxicillin treatment significantly reduced the number of lactobacilli (P <0.05) and Bifidobacterium (P <0.05) colonies in germ-free mice. However, the number of colonies colonized with amoxicillin in adult mice (53 days old) There was no significant difference in the control mice (P> 0.05). Conclusion Amoxicillin intervention during lactation will lead to the decrease of colonization rate of Lactobacillus and Bifidobacterium in mice, but the normal colonization rate can be reached after the mice are given off to adults. The mouse model of infant colonization can be simulated Existing animal models consistent amoxicillin Bifidobacterium, Lactobacillus colonization rules.