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[目的]探讨腺相关病毒2(AAV2)介导核转录因子KLF7对脱细胞异体神经支架(ANA)修复小鼠坐骨神经缺损后感觉轴突再生和感觉功能恢复的作用。[方法]成年C57BL/6小鼠随机分为正常组、ANA+AAV2对照组和ANA+AAV2-KLF组,术后4周RT-PCR和免疫荧光染色检测神经支架内KLF7 mRNA和蛋白表达,免疫荧光染色检测神经支架内NF和S100蛋白表达,霍乱毒素亚单位B结合荧光素TRITC(CTB-TRITC)逆行示踪L2~5脊髓背根神经节神经元(DRG)标记感觉轴突再生,Hargreaves法和电生理检测感觉功能的恢复。[结果]与对照组比较,ANA+AAV2-KLF组神经支架内KLF7mRNA和蛋白表达显著增高,NF和S100蛋白表达增加,CTB阳性标记DRG感觉神经元数量增加,Hargreaves热缩足反射潜伏期降低,电生理神经传导速度增加,潜伏时缩短,动作电位波幅增大(P<0.05)。[结论]KLF7可促进小鼠坐骨神经缺损后感觉轴突再生和髓鞘形成,促进感觉功能的恢复。
[Objective] To investigate the effect of adeno-associated virus 2 (AAV2) -mediated nuclear factor KLF7 on axonal regeneration and sensory recovery after sciatic nerve defect in mice with allogeneic nerve scaffold (ANA). [Methods] Adult C57BL / 6 mice were randomly divided into normal group, ANA + AAV2 control group and ANA + AAV2-KLF group. The expression of KLF7 mRNA and protein in nerve scaffolds was detected by RT-PCR and immunofluorescence staining four weeks after operation. Fluorescent staining was used to detect the expression of NF and S100 in neural scaffolds. Cholera toxin subunit B combined with CTB-TRITC retrograde tracing was used to detect sensory axon regeneration of L2 ~ 5 spinal dorsal root ganglion neurons (DRG) And electrophysiological detection of sensory function recovery. [Results] Compared with the control group, the expression of KLF7 mRNA and protein in the nerve scaffold of ANA + AAV2-KLF group was significantly increased, the expression of NF and S100 protein increased, the number of sensory neurons labeled CTB positive increased, the reflex latency of Hargreaves reduced, Physiological nerve conduction velocity increased, latency shortened, action potential amplitude increased (P <0.05). [Conclusion] KLF7 can promote axon regeneration and myelination after sciatic nerve defect in mice and promote the recovery of sensory function.