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建立了关于卡巴拉汀贴片的含量的高效液相色谱(HPLC)测定方法,采用离体的豚鼠皮肤作为透皮屏障,运用改进的Franz扩散池,观察卡巴拉汀透皮贴剂的体外透皮效果。结果显示,在9.69￣116.32μg/mL的浓度范围中卡巴拉汀的线性关系良好,精密度RSD是0.12%￣0.47%,检测限值是0.17pg/mL,低浓度的平均回收率为98.79%、中浓度为98.59%、高浓度为99.88%。实验中建立的HPLC方法具有准确、灵敏和操作简便的优点,对卡巴拉汀贴含量测定效果好。
A high performance liquid chromatography (HPLC) method for the determination of Rivastigmine patch was established. The guinea pig skin was used as transdermal barrier. The improved Franz diffusion cell was used to observe the in vitro permeability of Skin effect. The results showed that the linear relationship of rivastigmine was good in the concentration range of 9.69 ~ 116.32μg / mL with the precision RSD of 0.12% ~ 0.47%, the detection limit of 0.17pg / mL, the average recovery of low concentration was 98.79% , Medium concentration 98.59%, high concentration 99.88%. The HPLC method established in the experiment has the advantages of accurate, sensitive and easy to operate, and has good effect on the determination of rivastigmine.