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本实验从体内、体外两个方面初步探讨β内啡肽（βＥＰ）刺激ＩＬ１释放的机制。给正常大鼠静注不同浓度的βＥＰ，血浆ＩＬ１活性显著升高，呈剂量效应关系；给正常大鼠静注βＥＰ及ＬＰＳ，血浆ＩＬ１活性显著高于二者的单独作用；将不同浓度的βＥＰ与巨噬细胞一起培养（不加ＬＰＳ），培养液中呈现ＩＬ１活性，并在一定范围内与βＥＰ浓度有剂量效应关系；以低浓度的βＥＰ与巨噬细胞孵育１ｈ后去除βＥＰ，再加入ＬＰＳ，发现βＥＰ预处理组上清液ＩＬ１活性显著高于未加βＥＰ的对照组。结果提示：βＥＰ既能直接刺激巨噬细胞产生ＩＬ１，又能提高巨噬细胞对ＩＬ１诱生物ＬＰＳ的敏感性。
This experiment from the in vivo and in vitro aspects of two aspects of β endorphin (β EP) to stimulate IL 1 release mechanism. To normal rats intravenous injection of different concentrations of β EP, plasma IL 1 activity was significantly increased, the dose-response relationship; to normal rats intravenous β EP and LPS, plasma IL 1 activity was significantly higher than the two Single role; different concentrations of β EP cultured with macrophages (without LPS), the culture showed IL 1 activity and within a certain range and β EP concentration dose-response relationship; with low concentrations of β EP and macrophage incubated 1h after removal of β EP, then add LPS and found β EP pretreatment group supernatant IL 1 activity was significantly higher than without β EP control group. The results suggest that: β EP can directly stimulate macrophages to produce IL 1, but also improve the sensitivity of macrophages to IL 1 induced LPS.