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目的用高效液相色谱法测定清肺抑火丸中大黄酸、大黄素、大黄酚、大黄素甲醚的含量。方法 Dia-monsil-C18柱,甲醇-0.2%磷酸(85∶15);流速为1.0 mL.min-1,检测波长254 nm,柱温为室温。结果大黄酸、大黄素、大黄酚、大黄素甲醚的平均回收率分别为100.7%、97.4%、104.0%、103.6%,RSD分别为2.10%(n=6)、1.73%(n=6)、0.12%(n=6)、1.04%(n=6)。结论该法可用于清肺抑火丸的质量控制。
Objective To determine the content of rhein, emodin, chrysophanol and physcion in Qingfeiyanhuo pill by high performance liquid chromatography. Method Dia-monsil-C18 column, methanol-0.2% phosphoric acid (85:15). The flow rate was 1.0 mL.min-1. The detection wavelength was 254 nm and the column temperature was room temperature. Results The average recoveries of rhein, emodin, chrysophanol and physcion were 100.7%, 97.4%, 104.0% and 103.6%, respectively. The RSDs were 2.10% (n = 6) and 1.73% (n = 6) , 0.12% (n = 6), 1.04% (n = 6). Conclusion This method can be used for quality control of Qingfeiyuhuo Pills.