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目的以小鼠表皮神经酰胺含量、表皮厚度和组织病理变化为指标,对胶带法和丙酮乙醚法建立的皮肤机械屏障功能障碍模型进行评价。方法用胶带法和丙酮乙醚法分别破坏小鼠皮肤角质层,连续造模5 d后观察皮肤神经酰胺含量、表皮厚度和组织病理的变化。结果模型组小鼠表皮神经酰胺含量和表皮厚度均低于空白组,并出现相应的组织病理学改变,以胶带造模组改变更明显。结论两种造模方法均能造成小鼠的皮肤机械屏障功能障碍,其中以胶带法所建立的皮肤机械屏障功能障碍模型更稳定,操作更方便。
OBJECTIVE: To evaluate the model of cutaneous mechanical barrier dysfunction caused by tape method and acetone-ethyl ether method using the content of ceramide, epidermal thickness and histopathological changes in mouse epidermis as indexes. Methods The stratum corneum of mouse skin was destroyed by tape method and acetone ethyl ether method respectively. Changes of ceramide content, epidermal thickness and histopathology were observed after 5 days of continuous modeling. Results The content of epidermal ceramide and the thickness of epidermis in model group were lower than that in blank group, and the corresponding histopathological changes appeared. The changes in tape model group were more obvious. Conclusion Both models can cause mechanical barrier dysfunction in mice. The model of mechanical barrier dysfunction established by tape method is more stable and more convenient to operate.